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PrimerDesign Inc primer and probe selection software programs
Primer And Probe Selection Software Programs, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+selection+software+program/probe+and+primer+design/10__1016_slash_j__clinmicnews__2012__03__003-88-9-0
Average 90 stars, based on 1 article reviews
primer and probe selection software programs - by Bioz Stars, 2026-11
90/100 stars

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Article Title: qPCR quantification of Carnobacterium maltaromaticum, Brochothrix thermosphacta, and Serratia liquefaciens growth kinetics in mixed culture.
Article Snippet: Quantifying growth kinetics of specific spoilage microorganisms in mixed culture is required to describe the evolution of food microbiomes.. A qPCR method was developed to selectively amplify individual meat spoilage bacteria, Carnobacterium maltaromaticum, Brochothrix thermosphacta and Serratia liquefaciens, within a broth medium designed to simulate the composition of beef.. An optimized method of DNA extraction was produced for standard curve construction.

Article Title: Injection-mediated transposon transgenesis in Xenopus tropicalis and the identification of integration sites by modified extension primer tag selection (EPTS) linker-mediated PCR.
Article Snippet: The generation of transgenic lines is vital to many genetic strategies and provides useful reagents for cell labeling and lineage-tracing experiments.. Transposon-based systems offer simple, yet robust, platforms for transgenesis in the frog.. Here, we provide a protocol for a microinjection-based transposon transgenesis method using a ‘natural breeding’ strategy for the collection of Xenopus tropicalis embryos.

Selection:

Article Title: Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis.
Article Snippet: 2.5 Quantitative Real Time PCR Analysis of mRNA Levels Quantitative real-time PCR (qRT-PCR) was performed using an IQ5 instrument (Bio-Rad, UK) and the iScript One-Step RT-PCR Kit (Bio-Rad, UK) as described by Marchant et al., using the following primers; GCLm Forward 5’-gtcagggagtttccagat-3’, Reverse 5’gtcagggagtttccagat-3’; GCLc Forward 5’-ccaattgttatggctttgagtgc-3’, Reverse 5’ccaattgttatggctttgagtgc-3’ and values were normalized against TAF-7 (Forward 5’cgactccctagaacatgatgaa-3, Reverse 5’-gatcttctcggtgtcga-3’), β-actin and YWHAZ (primers supplied by Primer Design, UK). .. These three genes had been selected from a panel of the ten housekeeping genes using geNorm selection software (Primer Design, UK) and were deemed to exhibit the most stable expression in the GS-CHO cell lines analyzed in the study. ..

Article Title: Cytogenetics and characterization of microsatellite loci for a South American pioneer tree species, Croton floribundus.
Article Snippet: Despite the recent advances in plant population genetic studies, the lack of information regarding pedigree, ploidy level, or mode of inheritance for many polyploids can compromise the analysis of the molecular data produced.. The aim of this study was to examine both microsatellite and cytogenetic characteristics of the pioneer tree Croton floribundus Spreng. (Euphorbiaceae) to test for the occurrence of polyploidy in the species and to evaluate its implications for the appropriate use of SSR markers.. Seven microsatellite markers were developed and screened for 62 individuals from a semi-deciduous tropical forest in Brazil.

Article Title: Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis.
Article Snippet: Quantitative real-time PCR (qRT-PCR) was performed using an IQ5 instrument (Bio-Rad, UK) and the iScript One-Step RT-PCR Kit (Bio-Rad, UK) as described by Marchant et al.,34 using the following primers; GCLm Forward 5’-gtcagggagtttccagat-3’, Reverse 5’- gtcagggagtttccagat-3’; GCLc Forward 5’-ccaattgttatggctttgagtgc-3’, Reverse 5’- ccaattgttatggctttgagtgc-3’ and values were normalized against TAF-7 (Forward 5’- cgactccctagaacatgatgaa-3, Reverse 5’-gatcttctcggtgtcga-3’), β-actin and YWHAZ (primers supplied by Primer Design, UK). .. These three genes had been selected from a panel of the ten housekeeping genes using geNormTM selection software (Primer Design, UK)35 and were deemed to exhibit the most stable expression in the GS-CHO cell lines analyzed in the study. ..

Software:

Article Title: Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis.
Article Snippet: 2.5 Quantitative Real Time PCR Analysis of mRNA Levels Quantitative real-time PCR (qRT-PCR) was performed using an IQ5 instrument (Bio-Rad, UK) and the iScript One-Step RT-PCR Kit (Bio-Rad, UK) as described by Marchant et al., using the following primers; GCLm Forward 5’-gtcagggagtttccagat-3’, Reverse 5’gtcagggagtttccagat-3’; GCLc Forward 5’-ccaattgttatggctttgagtgc-3’, Reverse 5’ccaattgttatggctttgagtgc-3’ and values were normalized against TAF-7 (Forward 5’cgactccctagaacatgatgaa-3, Reverse 5’-gatcttctcggtgtcga-3’), β-actin and YWHAZ (primers supplied by Primer Design, UK). .. These three genes had been selected from a panel of the ten housekeeping genes using geNorm selection software (Primer Design, UK) and were deemed to exhibit the most stable expression in the GS-CHO cell lines analyzed in the study. ..

Article Title: Cytogenetics and characterization of microsatellite loci for a South American pioneer tree species, Croton floribundus.
Article Snippet: Despite the recent advances in plant population genetic studies, the lack of information regarding pedigree, ploidy level, or mode of inheritance for many polyploids can compromise the analysis of the molecular data produced.. The aim of this study was to examine both microsatellite and cytogenetic characteristics of the pioneer tree Croton floribundus Spreng. (Euphorbiaceae) to test for the occurrence of polyploidy in the species and to evaluate its implications for the appropriate use of SSR markers.. Seven microsatellite markers were developed and screened for 62 individuals from a semi-deciduous tropical forest in Brazil.

Article Title: Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis.
Article Snippet: Quantitative real-time PCR (qRT-PCR) was performed using an IQ5 instrument (Bio-Rad, UK) and the iScript One-Step RT-PCR Kit (Bio-Rad, UK) as described by Marchant et al.,34 using the following primers; GCLm Forward 5’-gtcagggagtttccagat-3’, Reverse 5’- gtcagggagtttccagat-3’; GCLc Forward 5’-ccaattgttatggctttgagtgc-3’, Reverse 5’- ccaattgttatggctttgagtgc-3’ and values were normalized against TAF-7 (Forward 5’- cgactccctagaacatgatgaa-3, Reverse 5’-gatcttctcggtgtcga-3’), β-actin and YWHAZ (primers supplied by Primer Design, UK). .. These three genes had been selected from a panel of the ten housekeeping genes using geNormTM selection software (Primer Design, UK)35 and were deemed to exhibit the most stable expression in the GS-CHO cell lines analyzed in the study. ..

Expressing:

Article Title: Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis.
Article Snippet: 2.5 Quantitative Real Time PCR Analysis of mRNA Levels Quantitative real-time PCR (qRT-PCR) was performed using an IQ5 instrument (Bio-Rad, UK) and the iScript One-Step RT-PCR Kit (Bio-Rad, UK) as described by Marchant et al., using the following primers; GCLm Forward 5’-gtcagggagtttccagat-3’, Reverse 5’gtcagggagtttccagat-3’; GCLc Forward 5’-ccaattgttatggctttgagtgc-3’, Reverse 5’ccaattgttatggctttgagtgc-3’ and values were normalized against TAF-7 (Forward 5’cgactccctagaacatgatgaa-3, Reverse 5’-gatcttctcggtgtcga-3’), β-actin and YWHAZ (primers supplied by Primer Design, UK). .. These three genes had been selected from a panel of the ten housekeeping genes using geNorm selection software (Primer Design, UK) and were deemed to exhibit the most stable expression in the GS-CHO cell lines analyzed in the study. ..

Article Title: Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis.
Article Snippet: Quantitative real-time PCR (qRT-PCR) was performed using an IQ5 instrument (Bio-Rad, UK) and the iScript One-Step RT-PCR Kit (Bio-Rad, UK) as described by Marchant et al.,34 using the following primers; GCLm Forward 5’-gtcagggagtttccagat-3’, Reverse 5’- gtcagggagtttccagat-3’; GCLc Forward 5’-ccaattgttatggctttgagtgc-3’, Reverse 5’- ccaattgttatggctttgagtgc-3’ and values were normalized against TAF-7 (Forward 5’- cgactccctagaacatgatgaa-3, Reverse 5’-gatcttctcggtgtcga-3’), β-actin and YWHAZ (primers supplied by Primer Design, UK). .. These three genes had been selected from a panel of the ten housekeeping genes using geNormTM selection software (Primer Design, UK)35 and were deemed to exhibit the most stable expression in the GS-CHO cell lines analyzed in the study. ..



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Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR <t>Lasergene</t> <t>12</t> software
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Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR <t>Lasergene</t> <t>12</t> software
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Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software

Journal: Plant Direct

Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

doi: 10.1002/pld3.60

Figure Lengend Snippet: Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software

Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

Techniques: Sequencing, Amplification, Software

Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph

Journal: Plant Direct

Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

doi: 10.1002/pld3.60

Figure Lengend Snippet: Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph

Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

Techniques: Sequencing, Software

Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software

Journal: Plant Direct

Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

doi: 10.1002/pld3.60

Figure Lengend Snippet: Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software

Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

Techniques: Sequencing, Generated, Amplification, Clone Assay, Software